Laser microprobe mass spectrometry of quaternary phosphonium salts: Direct versus matrix-assisted laser desorption

Laser microprobe mass spectrometry of quaternary phosphonium salts: Direct versus matrix-assisted laser desorption.

The use of laser microprobe mass spectrometry (LMMS) for the structural characterization of thermolabile quaternary phosphonium salts has been evaluated. A comparison has been made between LM mass spectra obtained by direct analysis of "neat" organic salts and the corresponding "matrix-assisted" LM mass spectra. Main limitations of LMMS for the direct analysis of neat organic salts (i.e., no ma...

Application of Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry

The clarification of metabolic dynamics in lesion areas is important. Many approaches, such as high performance liquid chromatography mass spectrometry, gas chromatography mass spectrometry, immunohistochemistry, are used to define disease-related abnormalities. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is attracting attention as a new valuable tool. MALD...

Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry

A new matrix assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) method to spatially profile the location and distribution of multiple N-linked glycan species in tissues is described. Application of an endoglycosidase, peptide N-glycosidase F (PNGaseF), directly on tissues followed by incubation releases N-linked glycan species amenable to detection by MALDI-IMS. The meth...

Laser desorption and matrix-assisted laser desorption/ionization mass spectrometry of 29-kDa Au:SR cluster compounds.

Positive and negative ions generated by laser-based ionization methods from three gold:thiolate cluster compounds are mass analyzed by time-of-flight mass spectrometry. The three compounds have similar inorganic core masses ( approximately 29 kDa, approximately 145 Au atoms) but different n-alkanethiolate ligands associated with each cluster compound (Au:SR, R = butane, hexane, dodecane). Irrad...

matrix-assisted laser desorption/ionization time of flight mass spectrometry: a new guide to infectious disease